Sarcoma cancer number

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Source: Fiziologia - Physiology. Abstract: Background: The adult mesenchymal stem cells MSCs are one of the most studied populations of adult multipotent stem cells, being isolated from bone marrow, peripheral blood, or other solid tissues.

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The fetal MSCs are isolated from placenta, amniotic fluid, umbilical cord and umbilical cord blood. Although they do not have the same proliferative and differentiation potentials of ESCs, the placenta-derived multipotent cells may be superior to adult stem cells, so that their increased potential should be explored in more detail for further therapeutic strategies.

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Materials and methods: Placental stem cells PSCs were isolated from 20 term-delivered placentas using enzymatic digestion Collagenase I A. Morphological characterization showed fibroblast-shape cellular appearance, adherent to plastic surface, and reaching confluence in spindle-like colonies. Tumor formation was completed in 4 weeks.

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For comparative analysis we used bone marrow-derived MSCs stably expressing GFP, and we visualized cellular migration, homing and effects by serial pictures taken every 3 days for 2 weeks using in vivo imaging system Hamamatsu Aequoria. Results: PSCs obtained using enzymatic digestion were similar to bone marrow-derived mesenchymal stem cells in morphological and functional characteristics. Conclusion: The present study shows that PSCs have an increased ability to modulate tumor growth compared to their counterparts, the bone marrow-derived MSCs, which makes them more suitable for therapeutic approaches in cancer treatment.

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